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HILIC for separation of co-eluted flavonoids under RP-HPLC mode
Authors:Zhang Hui  Guo Zhimou  Zhang Feifang  Xu Qing  Liang Xinmiao
Affiliation:Dalian Institute of Chemical Physics, Chinese Academy of Sciences Dalian, China.
Abstract:Hydrophilic interaction chromatography (HILIC) was employed to separate the co-eluted flavonoids from licorice extract under RP-HPLC mode. HILIC separations were carried out with the Atalantis HILIC Silica column and the CD-based column. The co-eluted flavonoids were well retained and separated on the two HILIC columns under HILIC mode. Similar results were obtained in the separation of another isoflavones sample, from kudzu extract under HILIC mode.
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