Study of the electrical connection mechanism of sheathless interface for capillary electrophoresis‐electrospray ionization‐mass spectrometry |
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Authors: | Cai Tie De‐Wen Zhang Hong‐Xu Chen Sen‐Lin Song Xin‐Xiang Zhang |
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Institution: | 1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Biochemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry, Peking University, , Beijing, 100871 China;2. Beckman Coulter Commercial Enterprise (China) Co., Ltd, 12/F., East Ocean Center, , Beijing, 100004 China;3. Beijing Center for Drug Abuser, Chinese Association of Drug Abuse Prevention and Treatment (CADAPT), , Daxing District, Beijing, 102600 China |
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Abstract: | With the combination of high separation ability of capillary electrophoresis (CE) and strong identification ability of mass spectrometry (MS), CE/MS is becoming a powerful tool for polar and ionic analytes analysis. Different interfaces have been developed to enhance the sensitivity and reliability since the first introduction of CE/MS in 1987. A sheathless porous interface based on a new ions transferring electric connection technique was reported to be with high sensitivity and reliability. In this work, a series of optical and electrochemical experiments were designed to study the electric connection process. The results indicated that closing CE electrical circuit and applying MS spray voltage were achieved by the small ions transferring through the interface porous wall. The new electric connection method significantly enhanced the sensitivity, resolution and stability of the CE/MS analysis. The interface was applied in CE/MS detection of morphine and 6‐monoacetylmorphine in urine sample and showed an equal sensitivity to LC/MS. With the significant improvement of sensitivity and stability, the CE/MS with the new interface showed strong potential for the determination of low abundance analytes. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | porous sheathless interface CE/MS electrical connection mechanism high sensitivity abused drug control |
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