|
|||||
|
|
Sucrose cryo‐protection facilitates imaging of whole eye sections by MALDI mass spectrometry |
|
| Authors: | Andrew D Palmer Rian Griffiths Iain Styles Ela Claridge Antonio Calcagni Josephine Bunch |
| Institution: | 1. PSIBS Doctoral Training Centre, University of Birmingham, , Birmingham, UK;2. School of Chemistry, University of Birmingham, , Birmingham, UK;3. School of Computer Science, University of Birmingham, , Birmingham, UK;4. School of Life and Health Science, Aston University, , Birmingham, UK |
| Abstract: | Sucrose is used as a cryo‐preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12‐µm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections. Copyright © 2012 John Wiley & Sons, Ltd. |
| Keywords: | MALDI Imaging Sucrose Cryo‐protection Eye Histology |