Recent trends in ultra‐fast HPLC: New generation superficially porous silica columns |
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Authors: | Imran Ali Zeid A AL‐Othman Norikaju Nagae Vinay D Gaitonde Kamlesh K Dutta |
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Institution: | 1. Department of Chemistry, Jamia Millia Islamia (Central University), , New Delhi, India;2. Department of Chemistry College of Science, King Saud University, , Riyadh, Kingdom of Saudi Arabia;3. ChromaNik Technologies Inc., , Minato‐ku Osaka, Japan;4. Prochrome India, , Andheri East, Mumbai, India;5. Quest Pharmaceuticals Pvt. Ltd., , Birgunj, Nepal |
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Abstract: | New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra‐fast HPLC separations for a variety of compounds with moderate sample loading capacity and low back pressure. Chemistries of these columns are C8, C18, RP‐Amide, hydrophilic interaction liquid chromatography, penta fluorophenyl (PFP), F5, and RP‐aqua. Normally, the silica gel particles are of 2.7 and 1.7 μm as total and inner solid core diameters, respectively, with 0.5‐μm‐thick of outer porous layer having 90 Å pore sizes and 150 m2/g surface area. This article describes these new generation columns with special emphasis on their textures and chemistries, separations, optimization, and comparison (inter and intra stationary phases). Besides, future perspectives have also been discussed. |
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Keywords: | Comparison New generation column Separation Superficially porous silica particle Ultra‐fast HPLC |
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