| 大蓟多糖提取分离及含量测定 |
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| 引用本文： | 吴彦,魏和平. 大蓟多糖提取分离及含量测定[J]. 光谱实验室, 2010, 27(1): 98-101 |
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| 作者姓名： | 吴彦 魏和平 |
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| 作者单位： | 安庆师范学院生命科学系,安徽省安庆市菱湖南路128号,246011;安庆师范学院生命科学系,安徽省安庆市菱湖南路128号,246011 |
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| 基金项目： | 安徽省教育厅自然科学重点研究项目(KJ2007A123ZC);;安徽省高等学校青年教师科研资助项目(2005jq1115) |
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| 摘 要： | 用水提-醇沉法从大蓟中提取多糖,脱脂、去蛋白,经冷冻干燥后得到粗多糖。通过苯酚-硫酸法测定糖的含量,粗多糖得率为6.5%。再采用DEAE-纤维素离子交换柱层析进一步分离纯化,得到5个次级组分DJ1,DJ2,DJ3,DJ4和DJ5。其中得率最高的是DJ2组分,为8.06‰。
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| 关 键 词： | 大蓟 多糖 提取 分离 离子交换柱层析 |
Extraction,Isolation and Determination of Polysaccharides from Cirsium Japonicum DC. |
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| WU Yan,WEI He-Ping. Extraction,Isolation and Determination of Polysaccharides from Cirsium Japonicum DC.[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(1): 98-101 |
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| Authors: | WU Yan WEI He-Ping |
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| Affiliation: | The Life Science Dept.of Anqing Normal College;Anqing;Anhui 246011;P.R.China |
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| Abstract: | The crude polysaccharide was extracted from cirsium japonicum DC.using hot waterethanol method to remove lipid and precipitate proteins.Its contents was determined by phenolsulphuric acid method after the sample was freeze-dried.The yield of the crude polysaccharide was 6.5%.The sample was further isolated into five subfractions(DJ1,DJ2,DJ3,DJ4 and DJ5)through ion-exchange chromatography of DEAE-cellulose.The highest yield is 8.06‰ from DJ2. |
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| Keywords: | Cirsium Japonicum DC. Polysaccharide Extraction Isolation Ion-Exchange Chromatography |
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