流动注射化学发光抑制法测定阿魏酸钠

提出了Fe2+-H2O2-亚甲基蓝化学发光新体系并用于阿魏酸钠的测定。实验发现,在酸性介质中,Fe2+-H2O2体系可与亚甲基蓝反应产生极强的化学发光,阿魏酸钠对此化学发光具有显著的抑制作用。据此,结合流动注射技术,建立了阿魏酸钠化学发光分析新方法。研究了影响化学发光强度的因素,化学发光信号的降低值(ΔI)与阿魏酸钠浓度在4.5×10-6～4.5×10-5mol/L范围内呈良好的线性关系,方法的检出限为7.0×10-7mol/L。对4.8×10-6mol/L的阿魏酸钠进行了11次平行测定,其RSD=0.8%,该法已用于片剂中阿魏酸钠含量的测定。

Determination of sodium ferulate by a flow-injection chemiluminescence inhibition method

A novel flow injection chemiluminescence (FI-CL) method for the determination of sodium ferulate was developed. It was observed that a strong chemiluminescence (CL) occurred when methylene blue solution was mixed with fenton reagent in an acidic medium, and the CL intensity greatly decreased with the addition of sodium ferulate into the reaction system. The decreased CL intensity was linear with the concentration of sodium ferulate in the range of 4.5×10~(-6)～4.5×10~(-5) mol/L under the optimum experimental conditions. The detection limit was 7.0×10~(-7) mol/L, and the relative standard deviation (RSD) was 0.8% for 4.8×10~(-6) mol/L sodium ferulate (n=11). This proposed method had been successfully applied to the determination of sodium ferulate in tablets.