Analysis of selected stilbenes in Polygonum cuspidatum by HPLC coupled with CoulArray detection |
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Authors: | Benová Blanka Adam Martin Onderková Katerina Královský Josef Krajícek Milan |
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Institution: | Research Institute of Organic Syntheses, Pardubice 20, Czech Republic. blanka.benova@email.cz |
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Abstract: | The roots of three varieties of Polygonum cuspidatum were analyzed for resveratrol and its analogs. The powder of the dried roots was extracted with aqueous ethanol (60% v/v) and the extracts obtained were analyzed using RP HPLC with coulometric detection. A simple HPLC method with a multichannel CoulArray detector was developed for the determination of four stilbenes: resveratrol, its glucoside piceid, piceatannol, and its glucoside astringin. Analyses were carried out on a LiChrospher C18 (125 x 4.6 mm id, particle size 5 microm) column with a mobile phase of ammonium acetate (pH 3) and ACN in gradient mode. Four compounds were monitored by a CoulArray electrochemical detector. Potentials of eight electrochemical cells in series were set in the range of 200-900 mV. Optimization of the mobile phase pH was performed. Calibration curves showed good linearity with correlation coefficients (r(2))--more than 0.9975. |
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Keywords: | Electrochemical detection HPLC Polygonum cuspidatum Stilbenes |
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