Application of negative ion MS/MS to the identification of N-glycans released from carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) |
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Authors: | Harvey David J Baruah Kavitha Scanlan Christopher N |
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Institution: | Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, UK. david.harvey@bioch.ox.ac.uk |
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Abstract: | Structures of N-glycans released from rat CEACAM1 expressed in human embryonic kidney cells were determined by MALDI and negative ion nanospray MS/MS techniques. The major carbohydrates were bi-, tri- and tetra-antennary complex glycans with and without sialic acid, fucose and bisecting GlcNAc residues. High-mannose glycans, predominantly Man(5)GlcNAc(2), were also found. The negative ion fragmentation technique easily identified the branching pattern of the triantennary glycans (mainly branched on the 6-antenna) and the presence of 'bisecting' GlcNAc residues (attached to the 4-position of the core mannose), features that are difficult to determine by traditional techniques. Sialic acids were in both alpha2-3 and alpha2-6 linkage as determined by MALDI-TOF MS following linkage-specific derivatization. |
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Keywords: | N‐Glycans negative ion electrospray isomer differentiation immunoglobulin G |
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