反相高效液相色谱法测定人参皂甙Compound-K的含量

人参皂甙compound-K(C-K)在人参中的含量极低,但它是其他含量较高的人参皂甙Rb1和Rb2等在人体肠道内的主要 降解产物和最终吸收形式,具有很高的生物活性。采用反相高效液相色谱法测定了人参总皂甙发酵液中C-K的含量。色谱 条件为:反相C18柱;乙腈-水(体积比为48∶52)溶液为流动相,流速1.0 mL/min;紫外检测波长203 nm;柱温35 ℃;外标法 定量。结果表明:C-K的质量浓度为0.05~0.8 g/L时,其峰面积与质量浓度具有良好的线性关系,相关系数为0.9998。方法 的检测限(S/N=3)为2.5 mg/L,峰面积测定值的相对标准偏差(n=6)为2.20%。测定栽培人参总皂甙及三七茎叶总皂甙微生 物发酵液中C-K的平均加标回收率(n=3)分别为98.6%和99.7%。该方法快速简便,准确可靠,可用于C-K的制备研究及药物 开发。

Determination of Ginsenoside Compound-K by Reversed-Phase High Performance Liquid Chromatography

Ginsenoside compound-K content in ginseng is very low, while it is the main intestinal bacterial metabolite and the final absorption style of the major components, such as ginsenosides Rbl and Rb2. The determination of ginsenoside compound-K in the fermentation liquor of ginseng saponins by reversed-phase high performance liquid chromatography was established. The separation was carried out under the following conditions: a Waters Symmetry C18 column (4.6 mm i.d. x 150 mm, 5 microm) was used at 35 degrees C with acetonitrile-water (48:52, v/v) as mobile phase at a flow rate of 1 mL/min. UV detection wavelength was set at 203 nm. The experimental results showed a good linear relationship between the peak area and mass concentration for ginsenoside compound-K within the range of 0.05-0.8 g/L (r = 0.9998). The relative standard deviation of peak area (n = 6) was 2.20%. The lowest detection limit (S/N = 3) was 2.5 mg/L. The average recoveries (n = 3) for the culture broth of ginseng saponins and notoginseng saponins were 98.6% and 99.7%, respectively. The method is rapid, simple, accurate and reproducible and can be utilized for the research and development of ginsenoside compound-K in pharmaceutical industry.