Differentiation of human serum samples by surface plasmon resonance monitoring of the integral glycoprotein interaction with a lectin panel |
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Authors: | Michael MecklenburgJuraj Svitel Fredrik WinquistJin Gang Katharina OrnsteinEstera Dey Xie BinEva Hedborg Ragnar NorrbyHans Arwin Ingemar LundströmBengt Danielsson |
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Institution: | a Pure and Applied Biochemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden b Department of Physics and Measurement Technology, Division of Applied Physics, Linköping University, SE-58183 Linköping, Sweden c Department of Infectious Diseases, Lund University Hospital, SE-22185 Lund, Sweden |
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Abstract: | Bacterial infection and inflammation result in massive changes in serum glycoproteins. These changes were investigated by the interaction of the saccharide glycoprotein moiety with lectins. A panel of eight lectins (Canavalia ensiformis, Bandeiraea simplicifolia BS-I, Arachis hypogaea, Phytolacca americana, Phaseolus vulgaris, Artocarpus integrifolia, Triticum vulgaris and Pisum sativum) was used to differentiate human serum glycoproteins obtained from patients with various bacterial infections. Lectin functionalised sensing layers were created on gold-coated wafers and lectin-glycoprotein interactions were monitored by surface plasmon resonance. The interaction of the lectin panel with serum glycoproteins produces unique patterns. Principal component analysis (PCA) was used to analyse the patterns. The actual panel of eight lectins enabled discrimination between sera obtained from patients sick with bacterial infection and healthy patients. Extended lectin panels have the potential to distinguish between types of bacterial infection and identify specific disease state. |
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Keywords: | Human serum Bacterial infection Glycoprotein Lectin panel Surface plasmon resonance Principal component analysis |
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