Positive contrast visualization for cellular magnetic resonance imaging using susceptibility-weighted echo-time encoding |
| |
Authors: | Young Beom Kim Ki Hyun Bae Seung-Schik Yoo Tae Gwan Park HyunWook Park |
| |
Institution: | 1. Division of Electrical Engineering, School of Electrical Engineering and Computer Science, KAIST, Daejeon 305-701, Republic of Korea;2. In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research, Cologne 50931, Germany;3. Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea;4. Department of Radiology, Harvard Medical School, Brigham and Women''s Hospital, Boston, MA 02115, USA |
| |
Abstract: | ObjectiveThe objective of this study was to investigate a method to generate positive contrast, selective to superparamagnetic iron oxide (SPIO) labeled cells, using the susceptibility-weighted echo-time encoding technique (SWEET).Materials and MethodsSPIO-labeled human epidermal carcinoma (KB) cells were placed in a gel phantom. Positive contrast from the labeled cells was created by subtraction between conventional spin-echo images and echo-time shifted susceptibility-weighted images. SPIO-labeled cells were injected into the left dorsal flank and hind limb of nude mice, and unlabeled cells were placed on the right side as controls. Tumor growth was monitored using the proposed method, and a histological analysis was used to confirm the presence of the labeled cells.ResultsBased on in vitro testing, we could detect 5000 labeled cells at minimum and the number of pixels with positive contrast increased proportionally to the number of labeled cells. Animal experiments also revealed the presence of tumor growth from SPIO-loaded cells.ConclusionsWe demonstrated that the proposed method, based on the simple principle of echo-time shift, could be readily implemented in a clinical scanner to visualize the magnetic susceptibility effects of SPIO-loaded cells through a positive-contrast mechanism. |
| |
Keywords: | Iron oxide particles Magnetic susceptibility Positive contrast In vivo cellular MRI Tumor |
本文献已被 ScienceDirect 等数据库收录! |
|