A coumarinylaldoxime as a specific sensor for Cu and its biological application |
| |
| Authors: | Olimpo Garcí a-Beltrá n,Bruce K. Cassels,Natalia Mena,Marco T. Nuñ ez,Osvaldo Yañ ez,Julio Caballero |
| |
| Affiliation: | 1. Department of Chemistry, Faculty of Sciences, University of Chile, Santiago, Chile;2. Facultad de Ciencias Naturales y Matemáticas, Universidad de Ibagué, Carrera 22 Calle 67, Ibagué, Colombia;3. Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile;4. Centro de Bioinformática y Simulación Molecular, Facultad de Ingeniería, Universidad de Talca, 2 Norte 685, Casilla 721, Talca, Chile |
| |
| Abstract: | In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi–Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed that JB interacts with the lipid bilayer at the level of the glycerol moieties. |
| |
| Keywords: | Cu2+-selectivity Fluorescent probe Coumarin derivative Molecular dynamics Lipid bilayer |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
