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Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe |
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| Authors: | Shengye Jin Jiping Chen Xinmiao Liang Xuhong Qian |
| Affiliation: | a Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China b Department of Fine Chemical Engineering, Dalian University of Technology, Dalian 116012, China c National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China |
| Abstract: | A flow injection system for the determination of organophosphate and carbamate pesticides is described. A sensitive fluorescence probe was synthesized and used as the pH indicator to detect the inhibition of the enzyme acetylcholinesterase (AChE). The percentage inhibition of enzyme activity is correlated to the pesticide concentration. Several parameters influencing the performance of the system are discussed. The detection limits of 3.5, 50, 12 and 25 μg/l for carbofuran, carbaryl, paraoxon and dichlorvos, in pure water, respectively were achieved with an incubation time of 10 min. A complete cycle of analysis, including incubation time, took 14 min. The detection system has been applied to the determination of carbofuran in spiked vegetable juices (Chinese cabbage and cole), achieving recovery values between 93.2 and 107% for Chinese cabbage juice and 108 and 118% for cole juice at the different concentration levels assayed. |
| Keywords: | Acetylcholinesterase Fluorescence probe Flow-injection analysis Organophosphate pesticides Carbamate pesticides Vegetable juices |
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