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Carbon Dots (C‐dots) from Cow Manure with Impressive Subcellular Selectivity Tuned by Simple Chemical Modification |
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| Authors: | Cintya D'Angelis do E. S. Barbosa Dr. José R. Corrêa Gisele A. Medeiros Gabrielle Barreto Dr. Kelly G. Magalhães Dr. Aline L. de Oliveira Prof. Dr. John Spencer Prof. Dr. Marcelo O. Rodrigues Prof. Dr. Brenno A. D. Neto |
| Affiliation: | 1. LIMA‐Laboratório de Inorganica e Materiais, Campus Universitário Darcy Ribeiro, CEP 70904‐970, P.O. Box 4478, Brasilia‐DF (Brazil), Also, Graduate Program from DQF‐UFPE;2. Laboratory of Medicinal and Technological Chemistry, Institute of Chemistry, University of Brasília, UnB, Campus Universitário Darcy Ribeiro, CEP 70904‐970, P.O. Box 4478, Brasília, DF (Brazil);3. Department of Chemistry, School of Life Sciences, University of Sussex, Falmer, Brighton, East Sussex, BN1 9QJ (UK) |
| Abstract: | Improved cellular selectivity for nucleoli staining was achieved by simple chemical modification of carbon dots (C‐dots) synthesized from waste carbon sources such as cow manure (or from glucose). The C‐dots were characterized and functionalized (amine‐passivated) with ethylenediamine, affording amide bonds that resulted in bright green fluorescence. The new modified C‐dots were successfully applied as selective live‐cell fluorescence imaging probes with impressive subcellular selectivity and the ability to selectively stain nucleoli in breast cancer cell lineages (MCF‐7). The C‐dots were also tested in four other cellular models and showed the same cellular selection in live‐cell imaging experiments. |
| Keywords: | carbon dots cell imaging fluorescence functionalized c‐dots nucleoli |