| In vitro TRANSFORMATION OF RAT ESOPHAGEAL EPITHELIAL CELLS BY FUSARIN C |
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| 作者姓名： | 鲁夫雄 李铭新 程书钧 |
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| 作者单位： | Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC |
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| 摘 要： | Fusarin C (FC) is a mutagenic and carcinogenic mycotoxin which was isolated from Fu-sarium moniliforme culture extracts. The Fusarium moniliforme is one of most prevalent fungi found on corn in Linxian, a high risk area for esophageal cancer. This paper reports, for the first time, the malignant transformation of rat esophageal epithelial cells induced by FC. The transformed cells showed several characteristics of transformation. Colonies were formed after seeding these transformed cells either into selective medium free of epidermal growth factor (EGF) and serum, or on semi-solid agar; there was an increase in chromosome number; the expression of c-myc and v-erb-B oncogenes was enhanced in the cells; and squamous cell carcinomas arose after inoculating the cells into nude mice. The results demonstrated transforming effect of FC on rat esophageal epithelial cells, and indicate that the abnormal expression of some oncogenes could serve as a hew property of transformed cells.
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In vitro TRANSFORMATION OF RAT ESOPHAGEAL EPITHELIAL CELLS BY FUSARIN C |
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| LU FU-XIONG LI MING-XIN CHENG SHU-JUN.In vitro TRANSFORMATION OF RAT ESOPHAGEAL EPITHELIAL CELLS BY FUSARIN C[J].Science in China(Chemistry),1991(12). |
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| Authors: | LU FU-XIONG LI MING-XIN CHENG SHU-JUN |
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| Abstract: | Fusarin C (FC) is a mutagenic and carcinogenic mycotoxin which was isolated from Fu-sarium moniliforme culture extracts. The Fusarium moniliforme is one of most prevalent fungi found on corn in Linxian, a high risk area for esophageal cancer. This paper reports, for the first time, the malignant transformation of rat esophageal epithelial cells induced by FC. The transformed cells showed several characteristics of transformation. Colonies were formed after seeding these transformed cells either into selective medium free of epidermal growth factor (EGF) and serum, or on semi-solid agar; there was an increase in chromosome number; the expression of c-myc and v-erb-B oncogenes was enhanced in the cells; and squamous cell carcinomas arose after inoculating the cells into nude mice. The results demonstrated transforming effect of FC on rat esophageal epithelial cells, and indicate that the abnormal expression of some oncogenes could serve as a hew property of transformed cells. |
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| Keywords: | rat esophagus epithelial cells malignant transformation Fusarin C oncogenes |
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