| Structure determination of glycopeptide of β-momorcharin |
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| 作者姓名： | 李一莉 马侃 吴厚铭 叶国杰 金善炜 汪猷 |
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| 作者单位： | LI，Yi-Li MA，Kan WU，Hou-MingYE，Guo-Jie JIN，Shan-Wei WANG，YuState Key Laboratory of Bio-Organic and Natural Products Chemistry，Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China |
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| 基金项目： | National Ministry of Science and Technology (No. 970211006)，Chinese Academy of Sciences (No.KJ951-A1-504-01). |
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| 摘 要： | The structure of the N-linked oligosaccharide chain of β-momorcharin, a ribosome-inactivating protein from the seeds of Momordica charantia Linn (Cucurbitaceae), was determined. A glycopeptide liberated by pronase digestion of the glycoprotein was subjected to amino acid and neutral carbohydrate analysis to establish the composition of amino acid and sugar residues. The sequences and glycosylation hnkages of the sugar and amino acid residues in the glycopeptide were determined as Manαl-6(XyIβ1-2)-Manβ1-4GlcNAcβ1-4(Fucαl-3)-GlcNAc-Asn-Leu by 2D-NMR spectroscopy and FAB-MS data.
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| 关 键 词： | β-Momorcharin ribosome-inactivating protein structure of glycopeptide. |
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