Ion-pair chromatography with UV absorbing counterions on reversed-phase layers. Part 1: Chromatography with hydroxynaphthalenesulfonic acids

The results of ion-pair chromatography with UV-absorbing counterions are described. Hydroxynaphthalenesulfonic acids are used as ion pair formers, and alkaloids as test samples. The separations are performed on silanized silica gel from Merck and KC₁₈F plates from Whatman. The influence of the counterion on the R_f-value is demonstrated. Some alkaloids exhibit characteristic fluorescence as ion pairs.