Tributyl phosphate degradation by immobilized cells of aCitrobacter sp. |
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Authors: | Owen S Jeong B C Poole P S Macaskie L E |
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Institution: | 1.Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Road, 0X1 3QU, Oxford, UK ;2.Department of Microbiology, University of Reading, London Road, Reading, RG1 5AQ, UK ;3.School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK ; |
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Abstract: | Tributyl phosphate (TBP), a plasticizer and solvent, is used in nuclear fuel reprocessing, generating TBP wastes laden with
residual uranium. ACitrobacter sp. accumulated heavy metals via a phosphohydrolase(s) that precipitated metals with inorganic phosphate liberated from an
organic phosphate “donor” molecule (TBP). Mutant analysis suggested that TBP hydrolysis was not attributable to a previously
documented acid phosphatase (monoesterase). Purified monoesterase had little activity against phospho di- and triesters, had
no requirement for Mg2+ or Mn2+, and was EDTA-resistant. Conversely, TBP cleavage by immobilized cells was enhanced by Mg2+, and ininhibited by Mn2+ and EDTA. A separate phosphotri/diesterase was implicated. |
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Keywords: | Index Entries" target="_blank">Index Entries Phosphoesterase tributyl phosphate biodegradation immobilized cells Citrobacter sp |
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