Effect of surface charge on adsorption of bovine serum albumin as studied by ellipsometry 1. Adsorption on cationic monolayer期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Effect of surface charge on adsorption of bovine serum albumin as studied by ellipsometry 1. Adsorption on cationic monolayer

Authors:	N. Watanabe T. Shirakawa M. Iwahashi K. Ohbu T. Seimiya

Affiliation:	(1) Present address: Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Tokyo, Japan;(2) Department of Chemistry, Faculty of Hygienic Science, Kitasato University, Kanagawa, Japan;(3) Applied Research Laboratories II, Lion Corporation, Tokyo, Japan

Abstract:	The adsorption of bovine serum albumin (BSA) onto a cationic monolayer (N,N-dimethyl-N,N-dialkylammonium chloride) spread at the air/water interface was studied by ellipsometry. Both thicknesses and refractive indices of the BSA layer adsorbed at the monolayer/solution interface are estimated from the observed change in phase difference and the ratio of reflection coefficients. The amount of adsorption of BSA resembles a Langmuir type isotherm. The adsorption changes with pH asymmetrically with respect to the pH of maximum adsorption, which was calculated to be 5.06 ± 0.47 mg/m². The amount of maximum adsorption implies that the BSA molecule adsorbs to the surface in a mode intermediate between side-on and end-on.

Keywords:	Ellipsometry bovine serum albumin protein adsorption cationic monolayer
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