应用于生物发酵在线监测的聚合物pH传感膜

微型生物反应器的成功操作依赖于对培养液pH值的监测和控制,聚合物pH荧光探针可以制成传感膜安装在微型反应器上,具有高通量筛选、非入侵性、容易集成等特征。甲基丙烯酸羟乙酯和N-(2-甲基丙烯酰乙酯基)-4-(N-甲基哌嗪基)-1,8-萘酰亚胺(NI)共聚形成的聚合物,具有良好亲水性、成膜性和生物相容性,并在pH=6~8之间具有良好的响应性能和稳定性。为了进一步提高膜的响应速率,将含有金刚烷的单体引入聚合物中,结果显示随着侧链金刚烷基团的含量增加,共聚物膜的pH响应时间变长;而将强亲水性的聚乙二醇引入共聚合物中,发现共聚物膜的pH响应速率得到了提高。将此pH传感膜应用于酵母发酵液中进行测试,具有良好的pH值在线监测性能。

Polymeric pH Sensor Film for Real-time Monitoring in Biological Culture

Successful operation of microbioreactors is critically dependent on the monitoring and control of pH of the culture fluids. Polymeric pH fluorescent probe can be easily fabricated into films and fixed onto microbioreactors. Copolymer P(HEMA-NI) has good hydrophilicity, film-forming property and biological compatibility, along with good response and repeatability in the pH range of 6-8 in the biological fermentation tank. In order to improve the response rate of pH polymer film, the third monomer was incorporated into the polymer matrix.The results showed that the pH response rate of films decreased with the incorporation of adamantine, while with the incorporation of hydrophilic PEG into the polymer, the response rate was significantly improved. These pH sensor films showed good pH on-line monitoring performance in yeast culture.