Catalytic DNA-and RNA-hydrolyzing antibodies from milk of healthy human mothers |
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Authors: | Valentina N Buneva Tat’yana G Kanyshkova Alexander V Vlassov Dmitry V Semenov Denis Yu Khumankov Lyubov’R Breusova Georgy A Nevinsky |
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Institution: | (1) Siberian Division of Russian Academy of Sciences, Novosibirsk Institute of Bioorgonic Chemistry, Lavrentieva Ave., 8, 630090 Novosibirsk, Russia;(2) Novosibirsk Specialized Hospital for Children, Novosibirsk, Russia |
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Abstract: | Various catalytically active antibodies (Abs), or abzymes, have been detected recently in the sera of patients with autoimmune
pathologies, in whom their presence is probably associated with autoimmunization. Normal humans are generally not considered
to have abzymes, since no obvious immunizing factors are present. Here is shown by different methods that IgG from the milk
of normal females possesses both DNase and RNase activities. The activities were also present in the IgG F(ab′)2 and Fab fragments.
Affinity modification of IgG by the chemically reactive derivative of an oligonucleotide led to preferential modification
of the L chain of IgG. After separation of the subunits by sodium dodecyl sulfate electrophoresis in a gel containing DNA,
an in-gel assay showed DNase activity in the L chain. The L chain separated by affinity chromatography on DNA-cellulose was
catalytically active. These findings speak in favor of the generation of cat alytic Abs by the immune system of healthy mothers.
It is known that the treatment of adults with DNases and RNases offers protection from viral and bacterial diseases. Since
breast milk protects the infants from infec tions until the immune system is developed, this raises the possibility that catalytic
Abs like nucleases, may possess a protective role. |
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Keywords: | Human milk DNase RNase IgG activities |
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