| 溴甲酚绿萃取分光光度法测定恩诺沙星 |
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| 引用本文： | 陈玉海,王晶,薛春丽,杨超,张彦青,刘保生.溴甲酚绿萃取分光光度法测定恩诺沙星[J].光谱实验室,2010,27(5):1830-1833. |
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| 作者姓名： | 陈玉海 王晶 薛春丽 杨超 张彦青 刘保生 |
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| 作者单位： | 1. 保定职业技术学院,河北省保定市朝阳南大街613号,071051;河北大学理化分析中心,河北省保定市五四东路180号,071002
2. 河北大学理化分析中心,河北省保定市五四东路180号,071002 |
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| 摘 要： | 在pH=2.0的盐酸-乙酸钠缓冲溶液中,恩诺沙星与溴甲酚绿反应生成1∶2的离子对缔合物,可被1,2-二氯乙烷萃取,其最大吸收波长为415nm。恩诺沙星的浓度在0.25—4.0mg/L范围内服从比耳定律,线性方程为A=0.4698C(mg/L)-0.0136,方法检出限为49.2μg/L。平行6次测定样品相对标准偏差为0.99%—3.92%,回收率为94.3%—103%。用于药物中恩诺沙星的检测,简捷、快速,结果满意。
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| 关 键 词： | 恩诺沙星 溴甲酚绿 萃取 紫外分光光度法 |
Determination of Enrofloxacin by the Bromocresol Green Extraction-Spectrophotometric Method |
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| CHEN Yu-Hai,WANG Jing,XUE Chun-Li,YANG Chao,ZHANG Yan-Qing,LIU Bao-Sheng.Determination of Enrofloxacin by the Bromocresol Green Extraction-Spectrophotometric Method[J].Chinese Journal of Spectroscopy Laboratory,2010,27(5):1830-1833. |
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| Authors: | CHEN Yu-Hai WANG Jing XUE Chun-Li YANG Chao ZHANG Yan-Qing LIU Bao-Sheng |
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| Institution: | ba(Baoding Vocational and Technical College,Baoding,Hebei 071051,P.R.China)b(Analysis Center of Physics and Chemistry,Hebei University,Baoding,Hebei 071002,P.R.China) |
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| Abstract: | A method for determination of enrofloxacin by the extraction spectrophotometry was proposed.The ion pairs complex of enrofloxacin with bromocresol green can be extracted into 1,2-methylene chloride at pH 2.0 and had maximum absorption at 415nm.Detected concentration of enrofloxacin in the range of 0.25—4.0mg/LA=0.4698C（mg/L）-0.0136,r=0.9997] covered with Beer＇s law.The detection limit was 49.2μg/L.The parallel six determination samples relatively standard deviation was 0.99%—3.92%.The recovery of method was 94.3%—103%.The proposed method has been successfully applied to the determination of enrofloxacin in pharmaceutical formulations with satisfactory results,and this method is simple,convenient and quick. |
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| Keywords: | Enrofloxacin Bromocresol Green Extraction UV Spectrophotometry |
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