RAPID EQUILIBRATION OF OXYGEN IN FLUORESCENCE QUENCHING REACTIONS |
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| Authors: | Maurice R. Eftink Camillo A. Ghiron |
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| Affiliation: | Department of Chemistry, University of Mississippi, University, MS 38677, USA;Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA |
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| Abstract: | ![]()
A simple means is described for the rapid equilibration of oxygen in fluorescence quenching reactions. This involves bubbling the gas through experimental solutions. With this procedure, data for Stern-Volmer plots can be obtained in 1–2 h, without bubbling it takes approximately ten times as long to collect such data. |
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