Structure elucidation of β-carotene isomers by HPLC-NMR coupling using a C30 bonded phase |
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Authors: | Sabine Strohschein Matthias Pursch Heidrun Händel K Albert |
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Institution: | Universit?t Tübingen, Institut für Organische Chemie, Auf der Morgenstelle 18, D-72?076 Tübingen, Germany, DE
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Abstract: | The separation of cis/trans isomers of β-carotene has been performed with a C30 stationary phase employing 1H NMR spectroscopy as an on-line detection technique. 1D as well as 2D NMR spectra have been recorded in the stopped-flow
mode for the predominant chromatographic peaks. Structural assignment of the five identified isomers was performed via comparison
of simulated 1D 1H NMR spectra on the basis of the structures of β-carotene cis/trans isomers with the experimental data, and also by the analysis of the proton-proton connectivities in the 2D NMR spectra of
three isomers with the highest concentration. The chromatographic retention behaviour of the isomers agreed well with previously
reported data. The advantage of the applied hyphenated coupling technique compared to conventional off-line techniques lies
in the fact that chromatographic separation and NMR detection are performed in a closed system, so that reisomerization of
the separated compounds is inhibited.
Received: 29 May 1996 / Revised: 1 July 1996 / Accepted: 4 July 1996 |
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