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BODIPY/Nile‐Red‐Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity |
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| Authors: | Dr Zhigang Yang Youngjin Wi Young‐Min Yoon Peter Verwilst Joo Hee Jang Prof Tae Woo Kim Prof Chulhun Kang Prof Jong Seung Kim |
| Institution: | 1. Department of Chemistry, Korea University, Seoul, Korea;2. Key Laboratory of optoelectronic devices and systems of ministry of education and Guangdong province, College of optoelectronic engineering, Shenzhen University, Shenzhen, China;3. The School of East-West Medical Science, Kyung Hee University, Yongin, Korea |
| Abstract: | We synthesized a boron‐dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome. |
| Keywords: | endoplasmic reticulum fluorescence FRET membrane fluidity metabolic syndrome |