| 高效液相色谱法和蒸发光散射检测器分析大豆磷脂分子种 |
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| 引用本文： | 夏海涛,刘玉芬,薛红艳,陈红卫.高效液相色谱法和蒸发光散射检测器分析大豆磷脂分子种[J].应用化学,2003,20(3):246-0. |
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| 作者姓名： | 夏海涛 刘玉芬 薛红艳 陈红卫 |
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| 作者单位： | 齐齐哈尔大学，长春工业大学 |
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| 摘 要： | 高效液相色谱法和蒸发光散射检测器分析大豆磷脂分子种
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| 关 键 词： | 高效液相色谱法和蒸发光散射检测器分析大豆磷脂分子种 |
| 文章编号： | 1000-0518(2003)03-0246-04 |
| 收稿时间： | 2009-06-29 |
| 修稿时间： | 2002年7月24日 |
Analysis of Soybean Phospholipids Molecular Species by HPLC Using Evaporative Light Scattering Detector |
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| XIA Hai-Tao ,LIU Yu-Fen,XUE Hong-Yan.Analysis of Soybean Phospholipids Molecular Species by HPLC Using Evaporative Light Scattering Detector[J].Chinese Journal of Applied Chemistry,2003,20(3):246-0. |
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| Authors: | XIA Hai-Tao LIU Yu-Fen XUE Hong-Yan |
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| Institution: | XIA Hai-Tao *,LIU Yu-Fen,XUE Hong-Yan |
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| Abstract: | Soybean phospholipids were separated into phospholipids on a semi-preparative s ilica gel column using HPLC. Phosphatidylcholine(PC), phosphatidylethanolami ne(PE) and phosphatidylinositol(PI) fractions were collected. Their molecular sp ecies compositions were analysed on a revers phase Prodigy ODS(3) column and a g radient elution program performed with chloroform-methanol-acetonitrile-water as a flow phase. Phosphatidylcholine, phosphatidylethanolamine and phosphatidyl inositol were separated into 11, 9 and 10 molecular species respectively. The molecular species identities were assign ed from fatty acid compositions determined by gas chromatography(GC) . The method has advantages of simplicity and availability of solvents . |
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| Keywords: | soybean phospholipid molecular species high performance liquid chromatography ev aporative light scattering detector |
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