正交轴逆流色谱法分离标准蛋白质及实际样品

 以m(质量分数为 12 .5 %的PEG80 0 0 )∶m(质量分数为 2 5 %的磷酸氢二钾 ) =1∶1或m(质量分数为 12 .5 %的PEG80 0 0 )∶m(质量分数为 30 %的磷酸氢二钾 ) =1∶1为溶剂系统 ,用下相作流动相 ,上相作固定相 ,操作时采用5 0 0r/min的转速和 6 0mL/h的流动相流速 ,考察了正交轴逆流色谱法对标准蛋白质及羊肚菌糖蛋白和枸杞糖肽的分离情况。将结果与高效液相色谱分离结果相比较 ,前者在分离度损失不大的基础上提高了进样量 ,证明了其用于制备的有效性 ,从而为分离制备天然生物大分子提供了一个新方法。

Separation of Standard Proteins and Actual Samples Using Cross-Axis Counter Current Chromatography

A system of m (12.5% PEG8000): m (25% K2HPO4) = 1:1 was used to separate two kinds of standard proteins, glycoprotein in Morchella esculenta (L.) and glycoprotein in Lycium barbarum (L.), the upper phase was used as stationary phase, and the lower phase was used as mobile phase. The revolution speed was 500 r/min and the flow rate was 60 mL/h. In comparing with high performance liquid chromatography, cross-axis counter current chromatography can increase load capacity without loss of resolution. The advantage of this method was verified, indicating that cross-axis counter current chromatography was a useful method for biopolymer separation.