Production of ligninolytic enzymes for dye decolorization by cocultivation of white-rot fungi Pleurotus ostreatus and Phanerochaete chrysosporium under solid-state fermentation |
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Authors: | Verma Pradeep Madamwar Datta |
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Institution: | (1) Post-Graduate Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar, 388120 Gujarat, India |
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Abstract: | Lignocellulosic wastes such as neem hull, wheat bran, and sugarcane bagasse, available in abundance, are excellent substrates
for the production of ligninolytic enzymes under solid-state fermentation by white-rot fungi. A ligninolytic enzyme system
with high activity showing enhanced decomposition was obtained by cocultivation of Pleurotus ostreatus and Phanerochaete chrysosporium on combinations of lignocellulosic waste. Among the various substrate combinations examined, neem hull and wheat bran wastes
gave the highest ligninolytic activity. A maximum production of laccase of 772 U/g and manganese peroxidase of 982 U/g was
obtained on d 20 and lignin peroxidase of 656 U/g on d 25 at 28±1 °C under solid-state fermentation. All three enzymes thus
obtained were partially purified by acetone fractionation and were exploited for decolorizing different types of acid and
reactive dyes. |
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Keywords: | Neem hulls white-rot fungi solid-state fermentation ligninolytic enzymes lignocellulosic waste |
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