| DNA-过氧化聚吡咯生物复合膜传感器的分析应用 |
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| 引用本文： | 蒋晓华,刘伟强,陈建军.DNA-过氧化聚吡咯生物复合膜传感器的分析应用[J].高等学校化学学报,2007,28(3):450-452. |
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| 作者姓名： | 蒋晓华 刘伟强 陈建军 |
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| 作者单位： | 深圳清华大学研究院新材料与生物医药研究所,深圳,518057 |
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| 摘 要： | 在研究DNA与儿茶酚胺类分子之间相互作用的基础上, 以碳纤维电极(CFE)为基底, 制备了一种新型的DNA-过氧化聚吡咯(PPyox)生物复合膜传感器, 与单一的DNA或PPyox修饰层相比具有更高的灵敏度和选择性.
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| 关 键 词： | DNA生物传感器 过氧化聚吡咯 多巴胺 5-羟色胺 |
| 文章编号： | 0251-0790（2007）03-0450-03 |
| 收稿时间： | 2006-06-26 |
| 修稿时间： | 2006-06-26 |
Application of DNA-overoxidized Polypyrrole Composite Biosensor |
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| JIANG Xiao-Hua,LIU Wei-Qiang,CHEN Jian-Jun.Application of DNA-overoxidized Polypyrrole Composite Biosensor[J].Chemical Research In Chinese Universities,2007,28(3):450-452. |
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| Authors: | JIANG Xiao-Hua LIU Wei-Qiang CHEN Jian-Jun |
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| Institution: | Center for Advanced Materials &; Biotechnology, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, China |
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| Abstract: | A promising electrochemical microsensor was fabricated by electrochemical immobilization of DNA on a carbon fiber electrode(CFE) through an overoxidized polypyrrole(PPyox) template for simultaneous determination of serotonin(5-HT) and dopamine(DA). Such a nano-thickness DNA-PPyox biocomposite modification layer exhibited a superior selectivity and sensitivity towards these neuronal amines comparing with a simple PPyox or DNA coating. This microsensor gave three separated differential pulse voltammetric(DPV) peaks at 0.38, 0.20 and -0.02 V for 5-HT, DA and 25000-fold excess of ascorbic acid(AA). The utility of this composite biosensor was demonstrated by the determination of 5-HT content in healthy human blood serum and DA in its hydrochloric injection. |
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| Keywords: | DNA-based biosensor Overoxidized polypyrrole Dopamine Serotonin |
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