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Melting domain in proteins
Authors:G Rialdi  E Battistel
Institution:(1) Istituto di Studi Chimico Fisici di Macromolecole Sintetiche e Naturali, CNR, Via De Marini 6, 16149 Genova, Italy;(2) Enichem spa, Istituto Guido Donegani, 28100 Novara, Italy
Abstract:Recent progress in thermodynamic aspects of proteins, free or immobilized on solid support, are described. In agreement with results observed with Ribonuclease A 9], DSC analysis on α-chymotrypsin confirms a decoupling of melting domains with the immobilized protein in a large range ofpH.
Keywords:enzymes  microcalorimetry  protein immobilization  protein melting domains
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