Melting domain in proteins |
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Authors: | G Rialdi E Battistel |
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Institution: | (1) Istituto di Studi Chimico Fisici di Macromolecole Sintetiche e Naturali, CNR, Via De Marini 6, 16149 Genova, Italy;(2) Enichem spa, Istituto Guido Donegani, 28100 Novara, Italy |
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Abstract: | Recent progress in thermodynamic aspects of proteins, free or immobilized on solid support, are described. In agreement with results observed with Ribonuclease A 9], DSC analysis on α-chymotrypsin confirms a decoupling of melting domains with the immobilized protein in a large range ofpH. |
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Keywords: | enzymes microcalorimetry protein immobilization protein melting domains |
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