Optimization of a lateral flow immunoassay for the ultrasensitive detection of aflatoxin M1 in milk |
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| Authors: | Laura Anfossi Claudio BaggianiCristina Giovannoli Flavia BiagioliGilda D’Arco Gianfranco Giraudi |
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| Institution: | Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy |
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| Abstract: | A high sensitive immunoassay-based lateral flow device for semi-quantitatively determine aflatoxin M1 (AFM1) in milk was developed. Investigation and optimization of the competitor design and of the gold-labelling strategy allowed the attainment of the ultra-sensitive assessment of AFM1 contamination at nanograms per litre level (LOD 20 ng L−1, IC50 99 ng L−1), as requested by European regulations. A one order of magnitude detectability enhancement in comparison to previously reported gold colloid immunochromatographic assays for this toxin was obtained. |
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| Keywords: | Immunochromatographic assay Gold colloid Mycotoxins Food analysis |
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