Simultaneous Determination of Fluorescein, Rhodamine 6G and Rhodamine B in Turbid Solution by Polarization Variable-Angle Synchronous Fluorescence Spectrometry

Polarization variable-angle synchronous fluorescence spectrometry was proposed to determine samples in turbid solution. A mixture of fluorescein, rhodamine 6G and rhodamine B was used to evaluate the technique. The background caused by scattering light was decreased remarkably. The limits of detection were 0.6 ng/ml forfluorescein, 2.3 ng/ml for rhodamine 6G and 4. 1 ng/ml for rhodamine B, respectively.