| 天然丰度的N-磷酰化氨基酸的15N NMR 研究 |
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| 引用本文： | 王喆,冯玉萍,刘林园,单璐,赵玉芬.天然丰度的N-磷酰化氨基酸的15N NMR 研究[J].波谱学杂志,2009,26(4):512-517. |
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| 作者姓名： | 王喆 冯玉萍 刘林园 单璐 赵玉芬 |
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| 作者单位： | 生命有机磷化学及化学生物学教育部重点实验室，清华大学 化学系，北京 100084 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 采用15N-1H的2D HSQC、HMBC实验方法，测定了天然丰度的N-磷酰化氨基酸样品在溶液中的15N化学位移δN及偶合常数JN-P，JN-H. 实验表明：对于15N天然丰度样品，这是一种快速有效的实验方法. 研究发现：N-酰化后的氨基酸，其δN以及与氮原子直接相连的质子1H的化学位移均发生十分明显的高场位移，而偶合常数1JN-P，1JN-H的变化与化合物构型相关联 .
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| 关 键 词： | 核磁共振（NMR） 15N NMR 2D NMR 天然丰度N-磷酰化氨基酸 |
| 收稿时间： | 2009-03-24 |
Natural Abundance N-Phosphoryl Amino Acids Studied by ~(15)N NMR Spectroscopy |
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| WANG Zhe,FENG Yu-Ping,Liu-Lin-Yuan,SHAN Lu,ZHAO Yu-Fen.Natural Abundance N-Phosphoryl Amino Acids Studied by ~(15)N NMR Spectroscopy[J].Chinese Journal of Magnetic Resonance,2009,26(4):512-517. |
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| Authors: | WANG Zhe FENG Yu-Ping Liu-Lin-Yuan SHAN Lu ZHAO Yu-Fen |
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| Institution: | Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China |
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| Abstract: | Natural abundance N-phosphoryl amino acids were studied by ~(15)N NMR spectroscopy. ~(15)N NMR chemical shifts and coupling constants were determined by ~(15)N-~1H heteronuclear HSQC and HMBC techniques. The stereosensitive ~1J (~(15)N-~(31)P) and ~1J (~(15)N-~1H) coupling constants were used to analyze the geometry of nitrogen atoms in these compounds. The structural and ~(15)N NMR spectroscopic characteristics of N-phosphoryl amino acids were discussed. |
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| Keywords: | ~(15)NNMR 2DNMR NMR ~(15)N NMR 2D NMR natural abundance N-phosphoryl amino acids |
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