毛细管等电聚焦方法及其应用

讨论了毛细管等电聚焦中所涉及的问题，如分离机理、电渗、迁移方法、检测器及其应用。由于毛细管等电聚焦操作方式的多样性，使其可适用于不同的仪器条件。非交联丙烯酰胺涂层能很好地消除电渗和蛋白吸附；而采用未处理的毛细管时，动态涂敷纤维素类亲水聚合物对碱性和中性蛋白亦能取得较好的分离效果。电荷耦合器件成像检测器尚待进一步发展才能成为常用的检测工具。对于复杂样品来说，仍需解决的问题是保证在较宽的ｐＨ范围内ｐＩ的线性。

Capillary Isoelectric Focusing Method and Its Applications

Capillary isoelectric focusing (CIEF) is reviewed in this article with 64 references. Separation mechanism, manipulation of electroosmosis, mobilization methods, detectors and some problems associated with the determination of samples are discussed. Different methods for performing CIEF are evaluated. Non-cross-linked polyacrylamide coated capillary is recommended. As for untreated column, dynamical coating with cellulose polymers allows a residual electroosmotic flow to carry the focused zone to pass through the detector. Some applications, such as separation of transferrin isoform, human Hb variants, t-PA glycoform, are presented.