高效液相色谱法测定人血浆中奥氮平的浓度

 用高效液相色谱法测定了人血浆中奥氮平的浓度。色谱条件 :采用岛津LC 6A型高效液相色谱仪 ;色谱柱为ZorbaxODS (15 0mm× 4 6mmi d ,粒径 5 μm) ;流动相为V(5 0mmol/L磷酸钠缓冲液 ,pH 7 2 )∶V(甲醇 )∶V(乙腈 ) =12∶10∶3的溶液 ;检测波长为 2 70nm ;流速为 1 0mL/min ;柱温 40℃ ;灵敏度 0 0 0 5AUFS ;纸速 2mm/min。实验结果显示 ,在上述条件下 ,该方法的线性范围为 15 μg/L～ 12 0 0 μg/L(r =0 9988) ,最低检测限为 3μg/L ,血浆中奥氮平的平均回收率为 (97 0 2± 3 11) % ,测定结果的日内平均相对偏差为 3 86 % (n =15 ) 。

Analysis of Olanzapine in Human Plasma withReversed-Phase High Performance Liquid Chromatography

Olanzapine is a novel antipsychotic drug. Recently it has been applied to clinical research. For the determination of olanzapine in plasma a reversed-phase HPLC method has been established. An aqueous solution of 0.25% ascorbic acid was added into the plasma sample and kept at -20 degrees C. Olanzapine can be separated on a Zorbax ODS column with a mobile phase of 50 mmol/L sodium phosphate (pH 7.2)-methanol-acetonitrile(12:10:3, volume ratios) and detected at 270 nm. The flow rate was 1.0 mL/min. The linear range was 15 micrograms/L-1,200 micrograms/L, r = 0.9988. The limited concentration of detection was 3 micrograms/L. The average recovery was (97.02 +/- 3.11)%. The intra-day and inter-day coefficients of variation were 3.86% (n = 15) and 4.12% (n = 15) respectively. The method has been used to determine olanzapine concentration in patient's plasma. The data obtained show that the sensitivity and selectivity of this method are adequate for drug monitoring in clinical research.