| 微分脉冲极谱同时测定三种碱性氨基酸的研究 |
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| 引用本文： | 黄玉秀.微分脉冲极谱同时测定三种碱性氨基酸的研究[J].分析测试学报,1997,16(6):52-54. |
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| 作者姓名： | 黄玉秀 |
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| 作者单位： | 华南理工大学应用化学系!广州510641 |
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| 摘 要： | 应用微分脉冲极谱，在ｐＨ９．０ ＮａＢ４Ｏ７－ ＣＨ３ＣＨＯ－Ｃｏ２＋－ ＮａＯＨ组成的极谱底液，扫描电位－ ０．８０～－ １．６０ Ｖ、振幅 ２０ｍＶ、扫描速度５ｍＶ／ｓ、一滴汞周期２ｓ的条件下进行连续扫描测量三种碱性氨基酸，获得三个灵敏 度和分辨率均较高的极谱峰图。该法简便快速，稳定可靠，为测量各种蛋白质中的三种磁性氨基酸提供一种新途 径。
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| 关 键 词： | 微分脉冲 配位体 碱性氨基酸 极谱仪 |
Simultaneous Determination of Basic Amino Acids by Differential Pulse Polarography |
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| Huang Yuxiu.Simultaneous Determination of Basic Amino Acids by Differential Pulse Polarography[J].Journal of Instrumental Analysis,1997,16(6):52-54. |
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| Authors: | Huang Yuxiu |
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| Abstract: | The differential pulse polarographic detendnation of cobalt complexes with histidine,lysine and arginine was studied at pH 9.0 in a system of Na2 B4 O7 - acetaldehyde- NaOH. High sensitivity and resolution can be obtained under the following conditions. The sweeping potential was in the range of - 0. 8 - - 1.6 V,while the scanning speed was kept at 5 mV.s~-1 with a pulse period of 1 drop of mercury per 2 seconds. The pulse amplitude was 20 mV. |
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| Keywords: | Differential Pulse polarography Basic amino acid ligands |
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