高效离子排斥色谱法测定饮料中的糖精钠

提出了一种用高效离子排斥色谱法测定饮料中糖精钠的新方法。以ＩｏｎＰａｃ ＩＣＥ－ＡＳ ６柱为分离柱，０．１０ ｍｍｏｌ／ＬＨ２ＳＯ４＋甲醇（９＋１）为淋洗液，２０２ｎｍ波长下紫外检测。在１－１００ｍｇ／Ｌ范围内，糖精钠的含量与峰面积呈 良好线性关系。检出限为０．６０ｎｇ，灵敏度优于以往采用离子交换色谱－电导检测法的结果。在实验条件下，饮料 中常见有机酸以及其它人工合成甜味剂等均不产生干扰。方法用于饮料中糖精钠的测定，加标回收率为９８％～ １０５％。此外还比较了电导检测、紫外检测和经化学抑制系统后紫外检测等三种检测方式对测定灵敏度的影响。

Determination of Sodium Saccharin in Drinks by High Performance Ion Exclusion Chromatography

Sodium saccharin in drinks was determined by a new high performance ion exclusion chromatographic method,by using a Dionex IonPaC ICE-AS 6 colunmn,a 202 nm UV detector,and a mobile phase of 0.10 mmol/L H2SO4 methanol (9 1),with a linear range of 1-100mg/l,a detection limit of 0.60 ng. Common organic acids such as citric acid,malic acid and sweeteners such as sodium cyclamate, aspartame and acesulfare potassium do not interfere.The method has been to determine sodium saccharin in drinks with a recovery range of 98% - 105%.