Fluorescent biosensor using whole cells in an inorganic translucent matrix

An optical biosensor based on vegetal cells entrapped in an inorganic translucent matrix and fluorescence detection has been developed. The biosensor uses Chlorella vulgaris immobilized in a translucent support produced from sol-gel technology. The translucence of the structure enables the algal active layer to be placed directly in contact with the optical fibers for fluorescence detection. This configuration has many advantages over the use of an opaque support because no space between the optical fibers and the active layer is required to collect fluorescence. This reagentless biosensor allows determination of diuron as an anti-PSII herbicide and its long term activity is assessed.