CE with Field-Enhanced Stacking for Rapid and Sensitive Determination of Umbelliferone, Rutin and Aesculetin in Prunella vulgaris |
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Authors: | Xiaoli Hu Shuya Cui Xingguo Chen Zhide Hu |
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Institution: | 1. College of Chemistry and Chemical Engineering, Mianyang Normal University, 621000, Mianyang, People’s Republic of China 2. College of Chemistry and Chemical Engineering, Lanzhou University, 730000, Lanzhou, People’s Republic of China
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Abstract: | A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of umbelliferone, rutin and aesculetin in Prunella vulgaris is established for the first time. After optimization of the separation and concentration conditions, the three analytes can be separated within 6 min and quantified with high sensitivity. The method was useful for qualitative and quantitative analysis of umbelliferone, rutin and aesculetin in Prunella vulgaris with recovery of 97.5 to 105.3%. |
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