分子信标用于核酸连续复制过程的体外实时监测

利用分子信标核酸探针实时监测了核酸连续复制过程. 分子信标不仅作为模板参与复制反应, 而且同步将复制过程的信息转换为荧光信号, 实现复制过程的体外实时监测. 该方法不仅为DNA复制检测提供了一种实时研究手段, 而且为核酸复制动力学及与复制相关疾病的深入研究提供了一种新的思路.

Real-time Monitoring Continuous Replication of DNA Leading Strand in vitro Based on Molecular Beacon

Real-time monitoring of replication of DNA leading strand using molecular beacon in vitro was reported. DNA replication plays a pivotal role in life process. DNA replication is usually assayed by denatured gel electrophoresis and autoradiography, which are complex, time-consuming, and incapable of acquiring the dynamic data in real time. We employed molecular beacon as the probe and template for DNA replication and converted directly replication information into fluorescence signal. The replication information of DNA leading strand was obtained conveniently and accurately using molecular beacon. This method would provide a strategy for real-time monitoring DNA replication and for further research of replication dynamic data and some deseases associated with DNA replication.