A novel amperometric glucose biosensor based on poly(glycidyl methacrylate-co-(3-thienylmethylmethacrylate)) |
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Authors: | Osman Karagollu Mesut Gorur Ali Turkan Busra Sengez Fethiye Gode Faruk Yilmaz |
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Institution: | 1. Department of Chemistry, Faculty of Arts and Science, Suleyman Demirel University, 32260 Isparta, Turkey;2. Department of Chemistry, Faculty of Sciences, Istanbul Medeniyet University, 34720 Kadikoy, Istanbul, Turkey;3. Department of Chemistry, Gebze Institute of Technology, 41400 Gebze, Kocaeli, Turkey |
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Abstract: | Two novel glucose oxidase (GOx) enzyme electrodes based on the copolymer of glycidyl methacrylate with 3-thienylmethyl methacrylate (poly(GMA-co-MTM)) with and without polypyrrole (PPyr) coating were prepared and employed in the amperometric determination of glucose levels. The effect of PPyr coating on the electrode properties was investigated in detail. Cyclic voltammetry studies showed that electrical conductivity of electrode B with PPyr coating (poly(GMA-co-MTM)/GOx/PPyr) was substantially higher than that of electrode A (poly(GMA-co-MTM)/GOx). On the other hand, electrode A showed better results in terms of sensitivity (10 nA/mM), limit of detection (50.2 μM), and response time (5 s). Electrodes A and B gave linear responses to the glucose concentrations in the range of 2–20 and 2–14 mM, respectively. The ranges of linearity for both enzyme electrodes are sufficient for the determination of physiological glucose concentrations in human blood. Moreover, PPyr coating of electrode B did not result in further stabilization of the enzyme electrode. |
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