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2D FT-ICR MS of Calmodulin: A Top-Down and Bottom-Up Approach |
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| Authors: | Federico Floris Maria van Agthoven Lionel Chiron Andrew J. Soulby Christopher A. Wootton Yuko P. Y. Lam Mark P. Barrow Marc-André Delsuc Peter B. O’Connor |
| Affiliation: | 1.University of Warwick,Coventry,UK;2.CASC4DE,Illkirch-Graffenstaden,France;3.IGBMC,Illkirch-Graffenstaden,France |
| Abstract: | Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS) allows data-independent fragmentation of all ions in a sample and correlation of fragment ions to their precursors through the modulation of precursor ion cyclotron radii prior to fragmentation. Previous results show that implementation of 2D FT-ICR MS with infrared multi-photon dissociation (IRMPD) and electron capture dissociation (ECD) has turned this method into a useful analytical tool. In this work, IRMPD tandem mass spectrometry of calmodulin (CaM) has been performed both in one-dimensional and two-dimensional FT-ICR MS using a top-down and bottom-up approach. 2D IRMPD FT-ICR MS is used to achieve extensive inter-residue bond cleavage and assignment for CaM, using its unique features for fragment identification in a less time- and sample-consuming experiment than doing the same thing using sequential MS/MS experiments. Graphical Abstract ? |
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