| Mg2+与胆汁蛋白质相互作用研究 |
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| 引用本文： | 谢安建,姚成立,沈玉华. Mg2+与胆汁蛋白质相互作用研究[J]. 无机化学学报, 2005, 21(3): 413-416 |
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| 作者姓名： | 谢安建 姚成立 沈玉华 |
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| 作者单位： | 安徽大学化学化工学院,合肥,230039;南京大学配位化学国家重点实验室,南京,210093 |
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| 基金项目： | 国家自然科学基金资助项目(No.20031010，20371001) |
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| 摘 要： | 胆汁是一中性溶液,在胆汁固形物中,胆汁酸盐、胆固醇、磷脂、蛋白质、胆红素分别占总质量的65%、3%、20%、5%及1%。蛋白质是胆汁固形物中第四多成分,在胆汁的生理功能的发挥和胆结石的形成中起到重要的作用。研究表明,胆汁蛋白质的主要成分是糖蛋白,主要由胆囊粘膜上皮细胞产生
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| 关 键 词： | Mg2+； 胆汁蛋白质； 荧光光谱； 紫外可见光谱 |
| 文章编号： | 1001-4861(2005)03-0413-04 |
| 收稿时间： | 2004-09-01 |
| 修稿时间： | 2004-09-01 |
Study on the Interaction between Mg2+ and Biliary Protein |
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| XIE An-Jian,YAO Cheng-Li and SHEN Yu-Hua. Study on the Interaction between Mg2+ and Biliary Protein[J]. Chinese Journal of Inorganic Chemistry, 2005, 21(3): 413-416 |
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| Authors: | XIE An-Jian YAO Cheng-Li SHEN Yu-Hua |
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| Affiliation: | College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039;State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093,College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039;State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093 and College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039;State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093 |
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| Abstract: | Biliary protein was isolated and purified by ammonium sulfate fractionation and Sephadex G-75 gel filtration column chromatography. FTIR showed that was a glycoprotein. Its molecular weight was determined as 34.7 kD by MS. The interaction between Mg2+ and biliary protein was studied by fluorescence spectroscopy and UV-Visible spectroscopy. The result showed the quenching mechanism of the combination of Mg2+ with biliary protein was a static quenching procedure. The protein underwent conformation changing and unfolding, more hydrophobic residues would be exposed to solvents after the Mg2+ was added. |
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| Keywords: | Mg2+ biliary protein fluorescence spectroscopy UV-Visible spectroscopy |
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