Identification of epigenetic DNA modifications with a protein nanopore |
| |
| Authors: | Wallace Emma V B Stoddart David Heron Andrew J Mikhailova Ellina Maglia Giovanni Donohoe Timothy J Bayley Hagan |
| |
| Affiliation: | Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, UK OX1 3TA. |
| |
| Abstract: | Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
