低能离子注入E.coli K12的 HRS/IRR效应及recA 基因在其诱发中的作用

以MG1655（野生型）， LE392（recA-）和DH5α（recA-）3株E.coliK12菌株为材料， 研究了30 keV N+离子注入E.coli K12时HRS/IRR效应的诱发情况及recA 基因在其诱发中的作用。 结果显示: 小于10×1014ions/cm2低剂量离子注入大肠杆菌可诱发HRS/IRR效应； 30 keV N+离子注入MG1655， LE392菌株都可诱发HRS/IRR效应， 而在DH5α菌株中无法诱导 IRR效应。 recA-与HRS/IRR 效应相斥性表明recA 基因在HRS/IRR效应的诱发中发挥了重要作用。 The HRS/IRR in Escherichia coli had been investigated withE.coli K12 wild strain MG1655 irradiated by the 30 keV N+. The curve of the dose survival effect showed the 30 keV N+ ion could induce the HRS/IRR at the dose less than 10×1014 ions/cm2. Moreover， the effect of recA gene in HRS/IRR inducement had been researched with three E.coli K12 strains， wild strain MG1655， LE392 (recA+) and DH5α(recA-). The results showed the IRR was disappeared in recA- DH5α strain， but the HRS/IRR appeared in wild strain MG1655 and recA+ LE392， which suggested that recA  gene and the DNA damages & their repairs including recA gene were essential element in HRS/IRR inducement.

HRS/IRR in E.coli K12 Implanted by Low Energy Ion Beam and Effect of recA Gene on HRS/IRR

The HRS/IRR in Escherichia coli had been investigated withE.coli K12 wild strain MG1655 irradiated by the 30 keV N+. The curve of the dose survival effect showed the 30 keV N+ ion could induce the HRS/IRR at the dose less than 10×1014 ions/cm2. Moreover， the effect of recA gene in HRS/IRR inducement had been researched with three E.coli K12 strains， wild strain MG1655， LE392 (recA+) and DH5α(recA-). The results showed the IRR was disappeared in recA- DH5α strain， but the HRS/IRR appeared in wild strain MG1655 and recA+ LE392， which suggested that recA  gene and the DNA damages & their repairs including recA gene were essential element in HRS/IRR inducement.