Determination of salicylic acid and its metabolites in urine by derivative synchronous spectrofluorimetry |
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| Authors: | F Salinas A Mu?oz de la Pe?a I Durán-Merás M Soledad Durán |
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| Institution: | Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain. |
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| Abstract: | The simultaneous determination of salicylic acid in binary and/or ternary mixtures and its two main urinary metabolites is proposed. Mixtures of salicylic, salicyluric and gentisic acids are resolved by synchronous spectrofluorimetry, in combination with first-derivative measurements. The urine is extracted with diethyl ether in acid medium. Salicylic and salicyluric acids are re-extracted into glycine-sodium hydroxide buffer solution of pH 11.6 and determined at that pH, and salicylic and gentisic acids are re-extracted into boric acid-sodium hydroxide buffer solution of pH 8.5 and determined at pH 6. |
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