| 碱性果胶裂解酶基因的克隆及核苷酸序列分析 |
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| 引用本文： | 翟超,曹军卫.碱性果胶裂解酶基因的克隆及核苷酸序列分析[J].武汉大学学报(理学版),2001,47(2):195-199. |
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| 作者姓名： | 翟超 曹军卫 |
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| 作者单位： | 武汉大学 生命科学学院, |
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| 摘 要： |
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| 关 键 词： | 碱性果胶裂解酶 嗜碱芽孢杆菌 克隆 核苷酸序列分析 |
| 文章编号： | 0253-9888(2001)02-0195-05 |
| 修稿时间： | 2000年12月19 |
Cloning and Sequencing of a Alkaline Pectate Lyase Gene |
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| ZHAI Chao,CAO Jun-Wei.Cloning and Sequencing of a Alkaline Pectate Lyase Gene[J].JOurnal of Wuhan University:Natural Science Edition,2001,47(2):195-199. |
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| Authors: | ZHAI Chao CAO Jun-Wei |
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| Abstract: | A pectate lyase gene was cloned from genome library of Bacillus alcalophillus NTT33. A positive clone was screened in YC plate. A about 6.5 kb DNA fragment containing pectate lyase gene was sequenced. According to the analysis in PCgene,it was found that DNA sequence from 150\|1 160 bp was a ORF region coding 337 amino acid of a pectate lyase. The recombinant plasmid was identified by restriction enzyme analysis. This PelA had some regions that were homologous to a PelE from an alkaliphilic strain of Bacillus with 38% identity and a PelA from Azospirilluum irakense with 32% identity.So it was supposed to be a new pectate lyase gene.The predict pI of the Pel was 5.3,so it belonged to pelA superfamily according to the established Pel superfamily. |
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| Keywords: | alkaline petate lyase Bacillus alcalophillus gene cloning nucleotide sequcucing |
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