树枝状碳硅烷涂层电色谱柱的制备及其评价

利用碳硅烷树枝状大分子末端SiCl键的高反应活性和多官能团性,将β-环糊精固定到毛细管柱上,制备了一种新型的手性毛细管电色谱柱。本涂层柱性能稳定,使用寿命长,经过1个月的连续运行,柱效能损失率低于5%。优化分离条件,采取16kV分离电压、检测波长214nm、10cm位差进样7s和40mmol/L磷酸盐缓冲体系,分离扑尔敏、盐酸异丙嗪和苯丙胺手性异构体,其中G2P代柱子对扑尔敏分离柱效达到2.5×105Plates/m,分离度RS=1.43,基线分离,取得了较满意的分离效果。

Preparation and Evaluation of Capillary Electrophoresis Column Bonded with Carbosilane Dendrimers

A new chiral capillary electrophoresis column coated with carbosilane dendrimers with peripheral Si–Cl groups and β-cyclodextrin was prepared. The separation characteristics of stability and longevity of capillaries modified by coating carbosilane dendrimers and β-cyclodextrin (β-CD) were satisfactory, and the variation of separation efficiency was less than 5% after continuous running for a month. The optimum conditions include a running voltage of 16 kV, a UV detector wavelength of 214 nm, sample injection time of 7 s, and a phosphate buffer solution concentration of 40 × 10⁻³ M. Chlortrimeton, Hydrochloric Promethazine, and Benzedrine were selected as the separation model targets. The G2P modified column was especially effective in separating Chlortrimeton enantiomers. Under the specified conditions, the column efficiency was 2.5 × 10⁵ Plates m⁻¹ with a resolution (R_S) of 1.43 and baseline separation was obtained. The results were satisfactory.