Assay of Phenformin Hydrochloride in Pharmaceutics by Coupling with Sodium Nitroprusside |
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| Authors: | Min Wang Yan Zhang Quan‐Min Li |
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| Institution: | 1. College of Chemistry and Environmental Science, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan 453007, P. R. China;2. College of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang, Henan 453003, P. R. China |
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| Abstract: | A novel and simple spectrophotometric method for the determination of phenformin hydrochloride using sodium nitroprusside as chromogenic reagent is developed in this paper. The experiment indicates that the sodium nitroprusside can react with the phenformin hydrochloride in a basic solution to form a product with colored N‐nitrosamines. The stoichiometric ratio of phenformin hydrochloride and sodium nitroprusside is 1:3 in the product. The maximal absorption wavelength (λmax) of the product is 520 nm, ?520 = 2.2 × 103 Lmol?1cm?1. A Good linear relationship is obtained between the absorbance (A) and the concentration (C) of phenformin hydrochloride in a wide range of 0.20‐400 μg mL?1. The equation of the linear regression is A = 0.03461 + 0.00907C (μg mL?1) with a linear correlation coefficient of 0.9991. The detection limit is 0.13 μg mL?1, and the relative standard deviation (R.S.D.) is 0.19%. The method is successfully applied to the determination of phenformin hydrochloride in tablet forms, and satisfactory recoveries are obtained. |
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| Keywords: | Sodium nitroprusside Chromogenic agent Phenformin hydrochloride Spectrophotometry |
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