Characterization of 10 species ofMahonia by capillary electrophoresis |
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Authors: | Yi Li Xiuhong Ji Huwei Liu Yuning Yan Jiashi Li |
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Affiliation: | (1) Department of Chemistry, Peking University, 100871 Beijing, P.R. China;(2) Beijing University of Chinese Medicine, 100029 Beijing, P.R. China |
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Abstract: | ![]() Summary A simple, rapid and effective capillary electrophoresis method was developed for the characterization of 10 different species ofMahonia. A fingerprint of the extract of each species was constructed using a mixed buffer of borate and phosphate, containing methanol, pH 8.0. The effective electrophoretic mobility and % normalized area of each peak in electrophoregrams were evaluated to characterize various species. Three alkaloids: berberine, palmatine and jatrorrhizine were found in all 10 species. The CE technique appears to provide a powerful tool for the identification and quality control of plant drugs. |
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Keywords: | Capillary electrophoresis Mahonia Fingerprint, alkaloids Berberine, palmatine and jatrorrhizine |
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