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Characterization of 10 species ofMahonia by capillary electrophoresis
Authors:Yi Li  Xiuhong Ji  Huwei Liu  Yuning Yan  Jiashi Li
Affiliation:(1) Department of Chemistry, Peking University, 100871 Beijing, P.R. China;(2) Beijing University of Chinese Medicine, 100029 Beijing, P.R. China
Abstract:
Summary A simple, rapid and effective capillary electrophoresis method was developed for the characterization of 10 different species ofMahonia. A fingerprint of the extract of each species was constructed using a mixed buffer of borate and phosphate, containing methanol, pH 8.0. The effective electrophoretic mobility and % normalized area of each peak in electrophoregrams were evaluated to characterize various species. Three alkaloids: berberine, palmatine and jatrorrhizine were found in all 10 species. The CE technique appears to provide a powerful tool for the identification and quality control of plant drugs.
Keywords:Capillary electrophoresis   Mahonia   Fingerprint, alkaloids  Berberine, palmatine and jatrorrhizine
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